US Secretary of State Marco Rubio has announced that the US is immediately revoking visas issued to all South Sudanese passport holders due to the African nation refusing to accept its citizens who have been removed from the US.
Rubio, in a statement on Saturday, added that the US will also block any arriving citizens of South Sudan, the world's newest country, at US ports of entry.
He blamed "the failure of South Sudan's transitional government to accept the return of its repatriated citizens in a timely manner".
A cornerstone of President Donald Trump's immigration policy is removing unlawful migrants from the US, with the promise of "mass deportations".
"It is time for the Transitional Government of South Sudan to stop taking advantage of the United States," said Rubio.
"Every country must accept the return of its citizens in a timely manner when another country, including the United States, seeks to remove them," he added.
It comes as fears grow that South Sudan may again descend into civil war.
On 8 March, the US ordered all its non-emergency staff in South Sudan to leave as regional fighting broke out, threatening a fragile peace deal agreed in 2018.
South Sudanese in the US were previously granted Temporary Protected Status (TPS), which allows them to remain in the US for a set period of time.
TPS for South Sudanese in the US had been due to expire by 3 May.
South Sudan, the world's newest nation, gained independence in 2011 after seceding from Sudan.
But just two years later, following a rift between President Salva Kiir and Vice-President Riek Machar, the tensions erupted into a civil war, in which more than 400,000 people were killed.
A 2018 power-sharing agreement between the two stopped the fighting, but key elements of the deal have not been implemented – including a new constitution, an election and the reunification of armed groups into a single army.
Sporadic violence between ethnic and local groups has continued in parts of the country.
Since returning to office, the Trump administration has clashed with international governments over deportations of their nationals from the US.
In January, Colombian President Gustavo Petro barred two US military flights carrying deported migrants from landing in his South American country.
Petro relented after Trump promised to place crippling tariffs and sanctions on Colombia.